Poster presentation, P16

Official XXIst International Pigment Cell Conference website - 21-24 Sept 2011, Bordeaux - France | updated: September 04 2011

Endothelin receptor B2 mutation induces the suppression of proliferation and migration of melanoblasts from early embryogenesis in quail and chickens

SPEAKER T. Akiyama #whois submiter ?
AUTHOR(s) T. Akiyama, A. Shinomiya, K. Kinoshita, M. Mizutani, T. Namikawa, S. Ito, Y. Matsuda

A signal transduction system of endothelins (EDNs) and their receptors (EDNRs) is known to be one of the strong factors to affect melanization in vertebrates. Aves have EDNRB2 in addition to EDNRB, as a paralog of it. The purpose of this study is to clarify the function of EDNRB2 in Aves. The Japanese quail line, “panda” (s/s), with EDNRB2 mutation that was reported previously showed white plumage as a main feature. Recently, several chickens with white-plumage mutation were found in Japanese native colored lines. These white individuals were crossed with the “albino” (recessive albino; ca/ca) and linkage mapping analysis was performed using F2 progenies having black eyes. We determined two types of the mutations in EDNRB2. The first type was identified from “Minohiki” and “Uzurao” lines and the mutation site was Cys244Phe. The second was isolated from “Ehime-jidori” and “Uzurao” with Thr8Ile, Thr15Ale and Arg332His substitutions in EDNRB2. The plumage colors were complete white in the former and partial white with mottling pattern in the latter. When we observed melanoblast proliferation during development, all of these EDNRB2 mutants showed clear suppression of the melanization in the integument and feather buds at the early developmental stage. Also, the organ culture of neural crests of these mutants showed that the quite less number of melanoblasts appeared from tissues than those of wild types and these cells didn’t grow well even addition with EDN3. These results clearly demonstrated that EDNRB2 is an indispensable factor in melanoblast development and migration to the final locations in Aves. In conclusion, EDNRB and -B2 in Aves are seemed to share the roles of EDBRB in mammals and then EDNRB2 may play a role specifically on proliferation and migration of melanoblasts in their bodies.



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