Oral communication, CS14 / C61

Official XXIst International Pigment Cell Conference website - 21-24 Sept 2011, Bordeaux - France | updated: September 04 2011

To trypsinise, or not to trypsinise, that is the question

BACKGROUND: Several tissue grafting techniques are available for treatment of stable vitiligo but very few comparative studies are available. AIM: To compare efficacy of autologous non-cultured trypsinised melanocyte-keratinocyte transfer versus autologous non-cultured non-trypsinised melanocyte-keratinocyte transfer in stable vitiligo patches. METHOD: Patients having anatomically comparable multiple patches of vitiligo stable for at least 1 year were enrolled for the study. After taking written informed consent, a patch was treated with autologous trypsinised melanocyte keratinocyte suspension (Standard technique) and its anatomically comparable counterpart was treated with autologous non-trypsinised melanocyte keratinocyte suspension (Jodhpur technique). Patch was assessed at 1 week for infection and at 1 month, 3 month and finally at 6 month for pigmentation. The results of the surgery of both techniques at the end of 6 months were evaluated by scoring system for autologous transplantation methods for vitiligo by Gupta S. et al. RESULTS: 9 patients with 42 patches of stable vitiligo were treated, of which 21 were treated by Standard technique and the anatomically comparable 21 patches were treated by Jodhpur technique. The 21 patches treated with Standard method were graded as excellent: 6, good: 2, fair: 6, poor: 7 and anatomically comparable 21 patches treated with Jodhpur technique were graded as excellent: 5, good: 2, fair: 1, poor: 13 by the scoring system for autologous transplantation in vitiligo. On statistical evaluation of the individual scores of standard technique (Mean 11.05 ± 7.00) and Jodhpur technique (Mean = 7.5 ± 7.63) the p value = 0.0064 by the paired t test which was statistically significant. CONCLUSION: On comparison of the two techniques standard technique was found to be more efficacious than the Jodhpur technique.

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